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cdna ends race pcr  (TaKaRa)


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    TaKaRa cdna ends race pcr
    Cdna Ends Race Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+ends+race+pcr/SMARTer+RACE+5%E2%80%99%2F3%E2%80%99+Kit/pm41921286-53-23-32
    Average 99 stars, based on 2992 article reviews
    cdna ends race pcr - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album
    Article Snippet: .. The full-length cDNA sequence of SaAACT and SaHMGS genes was isolated by 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR with the SMARTer RACE cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer’s protocol. ..

    Article Title: Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta).
    Article Snippet: sent to Sangon Biotech Co. Ltd. (Shanghai, China) for sequencing. .. To determine the full-length of SjNR cDNA, the rapid amplification of cDNA ends (RACE) PCR was performed following the manufacturer’s instruction of a SMARTer RACE 5′/3′ Kit (Clontech, Mountain View, California, USA), with two primers 5-GSP and 3-GSP which were designed based on the sequence of SjNR cDNA fragment that was amplified by the primers SjNR-F/R. ..

    Article Title: Characterization and functional analysis of the interferon regulatory factor 5 (IRF5) from the GIFT Oreochromis niloticus.
    Article Snippet: The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is an economically important fresh water fish globally.. Tilapia lake virus (TiLV) causes TiLV disease (TiLVD) in GIFT with a high mortality rate.. In this study, interferon regulatory factor 5 (IRF5) was identified from GIFT, and its antiviral and immunoregulatory roles were explored.

    Article Title: Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.
    Article Snippet: The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88–DLA-12 and DLA-88–DLA-88L.. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far.. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs.

    Article Title: Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis
    Article Snippet: .. Prior to cloning the full-length coding sequence of C. zofingiensi s FAD genes, their transcription start sites were determined by 5′ rapid amplification of cDNA ends (RACE)-PCR using the SMARTer RACE 5′ Kit (Clontech, CA, USA) and the 5′ gene-specific primers (Additional file : Table S3). ..

    Isolation:

    Article Title: Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album
    Article Snippet: .. The full-length cDNA sequence of SaAACT and SaHMGS genes was isolated by 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR with the SMARTer RACE cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer’s protocol. ..

    Rapid Amplification of cDNA Ends:

    Article Title: Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album
    Article Snippet: .. The full-length cDNA sequence of SaAACT and SaHMGS genes was isolated by 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR with the SMARTer RACE cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer’s protocol. ..

    Article Title: Light-Induced Transcription in Zebrafish Targets Mitochondrial Function and Heme Metabolism
    Article Snippet: The sequences of single, predicted D-box and E-box sequences were mutated (4–6 bp each) using the Q5® SiteDirected Mutagenesis kit (Promega) with the aim of disrupting the enhancer function. .. Rapid Amplification of cDNA Ends (RACE) PCR (SMARTer RACE 5′/3′ Kit, Takara Bio (San Jose, CA, USA)) was used to amplify the 5′ and 3′ ends of the HLF-1, TEF-2, and Nfil3-1b sequences of the Somalian cavefish according to the manufacturer’s instructions. ..

    Article Title: Nuclear receptor estrogen-related receptor modulates antimicrobial peptide expression for host innate immunity in Tribolium castaneum.
    Article Snippet: Antimicrobial peptides (AMPs) are core components of innate immunity to protect insects against microbial infections.. Nuclear receptors (NRs) are ligand-dependent transcription factors that can regulate the expression of genes critical for insect development including molting and metamorphosis.. However, the role of NRs in host innate immune response to microbial infection remains poorly understood in Tribolium castaneum (T. castaneum).

    Article Title: Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta).
    Article Snippet: sent to Sangon Biotech Co. Ltd. (Shanghai, China) for sequencing. .. To determine the full-length of SjNR cDNA, the rapid amplification of cDNA ends (RACE) PCR was performed following the manufacturer’s instruction of a SMARTer RACE 5′/3′ Kit (Clontech, Mountain View, California, USA), with two primers 5-GSP and 3-GSP which were designed based on the sequence of SjNR cDNA fragment that was amplified by the primers SjNR-F/R. ..

    Article Title: Characterization and functional analysis of the interferon regulatory factor 5 (IRF5) from the GIFT Oreochromis niloticus.
    Article Snippet: The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is an economically important fresh water fish globally.. Tilapia lake virus (TiLV) causes TiLV disease (TiLVD) in GIFT with a high mortality rate.. In this study, interferon regulatory factor 5 (IRF5) was identified from GIFT, and its antiviral and immunoregulatory roles were explored.

    Article Title: Light-Induced Transcription in Zebrafish Targets Mitochondrial Function and Heme Metabolism
    Article Snippet: The sequences of single, predicted D-box and E-box sequences were mutated (4–6 bp each) using the Q5 ® Site-Directed Mutagenesis kit (Promega) with the aim of disrupting the enhancer function. .. Rapid Amplification of cDNA Ends (RACE) PCR (SMARTer RACE 5′/3′ Kit, Takara Bio (San Jose, CA, USA)) was used to amplify the 5′ and 3′ ends of the HLF-1, TEF-2, and Nfil3-1b sequences of the Somalian cavefish according to the manufacturer’s instructions. ..

    Article Title: Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.
    Article Snippet: The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88–DLA-12 and DLA-88–DLA-88L.. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far.. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs.

    Article Title: Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis
    Article Snippet: .. Prior to cloning the full-length coding sequence of C. zofingiensi s FAD genes, their transcription start sites were determined by 5′ rapid amplification of cDNA ends (RACE)-PCR using the SMARTer RACE 5′ Kit (Clontech, CA, USA) and the 5′ gene-specific primers (Additional file : Table S3). ..

    Amplification:

    Article Title: Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album
    Article Snippet: .. The full-length cDNA sequence of SaAACT and SaHMGS genes was isolated by 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR with the SMARTer RACE cDNA Amplification Kit (Clontech, Palo Alto, CA, USA) according to the manufacturer’s protocol. ..

    Article Title: Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta).
    Article Snippet: sent to Sangon Biotech Co. Ltd. (Shanghai, China) for sequencing. .. To determine the full-length of SjNR cDNA, the rapid amplification of cDNA ends (RACE) PCR was performed following the manufacturer’s instruction of a SMARTer RACE 5′/3′ Kit (Clontech, Mountain View, California, USA), with two primers 5-GSP and 3-GSP which were designed based on the sequence of SjNR cDNA fragment that was amplified by the primers SjNR-F/R. ..

    Article Title: Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.
    Article Snippet: The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88–DLA-12 and DLA-88–DLA-88L.. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far.. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs.

    Polymerase Chain Reaction:

    Article Title: Light-Induced Transcription in Zebrafish Targets Mitochondrial Function and Heme Metabolism
    Article Snippet: The sequences of single, predicted D-box and E-box sequences were mutated (4–6 bp each) using the Q5® SiteDirected Mutagenesis kit (Promega) with the aim of disrupting the enhancer function. .. Rapid Amplification of cDNA Ends (RACE) PCR (SMARTer RACE 5′/3′ Kit, Takara Bio (San Jose, CA, USA)) was used to amplify the 5′ and 3′ ends of the HLF-1, TEF-2, and Nfil3-1b sequences of the Somalian cavefish according to the manufacturer’s instructions. ..

    Article Title: Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta).
    Article Snippet: sent to Sangon Biotech Co. Ltd. (Shanghai, China) for sequencing. .. To determine the full-length of SjNR cDNA, the rapid amplification of cDNA ends (RACE) PCR was performed following the manufacturer’s instruction of a SMARTer RACE 5′/3′ Kit (Clontech, Mountain View, California, USA), with two primers 5-GSP and 3-GSP which were designed based on the sequence of SjNR cDNA fragment that was amplified by the primers SjNR-F/R. ..

    Article Title: Characterization and functional analysis of the interferon regulatory factor 5 (IRF5) from the GIFT Oreochromis niloticus.
    Article Snippet: The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is an economically important fresh water fish globally.. Tilapia lake virus (TiLV) causes TiLV disease (TiLVD) in GIFT with a high mortality rate.. In this study, interferon regulatory factor 5 (IRF5) was identified from GIFT, and its antiviral and immunoregulatory roles were explored.

    Article Title: Light-Induced Transcription in Zebrafish Targets Mitochondrial Function and Heme Metabolism
    Article Snippet: The sequences of single, predicted D-box and E-box sequences were mutated (4–6 bp each) using the Q5 ® Site-Directed Mutagenesis kit (Promega) with the aim of disrupting the enhancer function. .. Rapid Amplification of cDNA Ends (RACE) PCR (SMARTer RACE 5′/3′ Kit, Takara Bio (San Jose, CA, USA)) was used to amplify the 5′ and 3′ ends of the HLF-1, TEF-2, and Nfil3-1b sequences of the Somalian cavefish according to the manufacturer’s instructions. ..

    Article Title: Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.
    Article Snippet: The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88–DLA-12 and DLA-88–DLA-88L.. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far.. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs.

    cDNA Synthesis:

    Article Title: Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events.
    Article Snippet: The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures, DLA-88–DLA-12 and DLA-88–DLA-88L.. However, detailed information of the genomic differences among DLA-88, DLA-12, and DLA-88L are still lacking at the full-length gene level, and therefore, DLA allelic sequences classified for each of these loci are limited in number so far.. In this study, we determined the DNA sequence of a 95-kb DLA class I genomic region including DLA-88, DLA-12/88L, and DLA-64 with three DLA homozygous dogs and of 37 full-length allelic gene sequences for DLA-88 and DLA-12/88L loci in 26 DLA class I homozygous dogs.

    Cloning:

    Article Title: Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis
    Article Snippet: .. Prior to cloning the full-length coding sequence of C. zofingiensi s FAD genes, their transcription start sites were determined by 5′ rapid amplification of cDNA ends (RACE)-PCR using the SMARTer RACE 5′ Kit (Clontech, CA, USA) and the 5′ gene-specific primers (Additional file : Table S3). ..



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    ( A ) Genomic anatomy of lncIrf8 locus determined by 3’ and 5’ <t>RACE</t> <t>PCR.</t> Blue box, exon 2 and 3 (48 bp and 468 bp, respectively). The 1010 bp intron and polyA tail are shown. Data of RNA-seq, ATAC-seq, ChIP-seq of H3K27ac (enhancer mark) and H3K4me3 (active promoter mark, near TSS) are visualized by IGV browser for the indicated cell populations (pDC, all cDC, cDC1 and cDC2). Grey box, lncIrf8 promoter KO region; open box, cDC1 specific +32 kb enhancer by . Irf8 +32 kb enhancer based on the H3K27ac enhancer mark is indicated with a purple line. Scale bar: 1 kb. ( B ) Gene expression of lncIrf8 and Irf8 in lncIrf8 promoter KO and control at day 0, 5, and 7 of Flt3L directed DC differentiation. Gene expression was determined by RT-qPCR and normalized to GAPDH . n=4. ( C ) Representative flow cytometry analysis of Flt3L directed DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control ( ; ). pDC, all cDC, cDC1, and cDC2 were gated as in and are shown. Bar diagrams depict quantification of pDC, cDC1 and cDC2 normalized to living single cells on DC differentiation day 0, 3, 5, 7, and 9. n=6–7. ( D ) Representative phase-contrast microscopy images of lncIrf8 promoter KO HoxB8 MPP and control on day 7 of Flt3L directed DC differentiation. Scale bar: 200 μm. ( E ) Representative flow cytometry analysis of spontaneous DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control with growth factors but without E2 ( ; ) at day 8. Gr1 + monocytes and CD11c + DC are shown. Quantification of Gr1 + monocytes of living single cells on day 3, 6, 8, and 10 of spontaneous DC differentiation. n=6, lncIrf8 promoter KO; n=4, control. Empty gRNA vector or non-targeting gRNA vector HoxB8 MPP were used as controls. Data represent mean ± SD of at least three independent experiments with different HoxB8 MPP clones of lncIrf8 promoter KO and control without deletion. *p<0.05, **p<0.01, ***p<0.001, multiple t-tests. Data that have no difference (p>0.05) are not labeled.
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    ( A ) Genomic anatomy of lncIrf8 locus determined by 3’ and 5’ <t>RACE</t> <t>PCR.</t> Blue box, exon 2 and 3 (48 bp and 468 bp, respectively). The 1010 bp intron and polyA tail are shown. Data of RNA-seq, ATAC-seq, ChIP-seq of H3K27ac (enhancer mark) and H3K4me3 (active promoter mark, near TSS) are visualized by IGV browser for the indicated cell populations (pDC, all cDC, cDC1 and cDC2). Grey box, lncIrf8 promoter KO region; open box, cDC1 specific +32 kb enhancer by . Irf8 +32 kb enhancer based on the H3K27ac enhancer mark is indicated with a purple line. Scale bar: 1 kb. ( B ) Gene expression of lncIrf8 and Irf8 in lncIrf8 promoter KO and control at day 0, 5, and 7 of Flt3L directed DC differentiation. Gene expression was determined by RT-qPCR and normalized to GAPDH . n=4. ( C ) Representative flow cytometry analysis of Flt3L directed DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control ( ; ). pDC, all cDC, cDC1, and cDC2 were gated as in and are shown. Bar diagrams depict quantification of pDC, cDC1 and cDC2 normalized to living single cells on DC differentiation day 0, 3, 5, 7, and 9. n=6–7. ( D ) Representative phase-contrast microscopy images of lncIrf8 promoter KO HoxB8 MPP and control on day 7 of Flt3L directed DC differentiation. Scale bar: 200 μm. ( E ) Representative flow cytometry analysis of spontaneous DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control with growth factors but without E2 ( ; ) at day 8. Gr1 + monocytes and CD11c + DC are shown. Quantification of Gr1 + monocytes of living single cells on day 3, 6, 8, and 10 of spontaneous DC differentiation. n=6, lncIrf8 promoter KO; n=4, control. Empty gRNA vector or non-targeting gRNA vector HoxB8 MPP were used as controls. Data represent mean ± SD of at least three independent experiments with different HoxB8 MPP clones of lncIrf8 promoter KO and control without deletion. *p<0.05, **p<0.01, ***p<0.001, multiple t-tests. Data that have no difference (p>0.05) are not labeled.
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    Image Search Results


    ( A ) Genomic anatomy of lncIrf8 locus determined by 3’ and 5’ RACE PCR. Blue box, exon 2 and 3 (48 bp and 468 bp, respectively). The 1010 bp intron and polyA tail are shown. Data of RNA-seq, ATAC-seq, ChIP-seq of H3K27ac (enhancer mark) and H3K4me3 (active promoter mark, near TSS) are visualized by IGV browser for the indicated cell populations (pDC, all cDC, cDC1 and cDC2). Grey box, lncIrf8 promoter KO region; open box, cDC1 specific +32 kb enhancer by . Irf8 +32 kb enhancer based on the H3K27ac enhancer mark is indicated with a purple line. Scale bar: 1 kb. ( B ) Gene expression of lncIrf8 and Irf8 in lncIrf8 promoter KO and control at day 0, 5, and 7 of Flt3L directed DC differentiation. Gene expression was determined by RT-qPCR and normalized to GAPDH . n=4. ( C ) Representative flow cytometry analysis of Flt3L directed DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control ( ; ). pDC, all cDC, cDC1, and cDC2 were gated as in and are shown. Bar diagrams depict quantification of pDC, cDC1 and cDC2 normalized to living single cells on DC differentiation day 0, 3, 5, 7, and 9. n=6–7. ( D ) Representative phase-contrast microscopy images of lncIrf8 promoter KO HoxB8 MPP and control on day 7 of Flt3L directed DC differentiation. Scale bar: 200 μm. ( E ) Representative flow cytometry analysis of spontaneous DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control with growth factors but without E2 ( ; ) at day 8. Gr1 + monocytes and CD11c + DC are shown. Quantification of Gr1 + monocytes of living single cells on day 3, 6, 8, and 10 of spontaneous DC differentiation. n=6, lncIrf8 promoter KO; n=4, control. Empty gRNA vector or non-targeting gRNA vector HoxB8 MPP were used as controls. Data represent mean ± SD of at least three independent experiments with different HoxB8 MPP clones of lncIrf8 promoter KO and control without deletion. *p<0.05, **p<0.01, ***p<0.001, multiple t-tests. Data that have no difference (p>0.05) are not labeled.

    Journal: eLife

    Article Title: A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation

    doi: 10.7554/eLife.83342

    Figure Lengend Snippet: ( A ) Genomic anatomy of lncIrf8 locus determined by 3’ and 5’ RACE PCR. Blue box, exon 2 and 3 (48 bp and 468 bp, respectively). The 1010 bp intron and polyA tail are shown. Data of RNA-seq, ATAC-seq, ChIP-seq of H3K27ac (enhancer mark) and H3K4me3 (active promoter mark, near TSS) are visualized by IGV browser for the indicated cell populations (pDC, all cDC, cDC1 and cDC2). Grey box, lncIrf8 promoter KO region; open box, cDC1 specific +32 kb enhancer by . Irf8 +32 kb enhancer based on the H3K27ac enhancer mark is indicated with a purple line. Scale bar: 1 kb. ( B ) Gene expression of lncIrf8 and Irf8 in lncIrf8 promoter KO and control at day 0, 5, and 7 of Flt3L directed DC differentiation. Gene expression was determined by RT-qPCR and normalized to GAPDH . n=4. ( C ) Representative flow cytometry analysis of Flt3L directed DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control ( ; ). pDC, all cDC, cDC1, and cDC2 were gated as in and are shown. Bar diagrams depict quantification of pDC, cDC1 and cDC2 normalized to living single cells on DC differentiation day 0, 3, 5, 7, and 9. n=6–7. ( D ) Representative phase-contrast microscopy images of lncIrf8 promoter KO HoxB8 MPP and control on day 7 of Flt3L directed DC differentiation. Scale bar: 200 μm. ( E ) Representative flow cytometry analysis of spontaneous DC differentiation of lncIrf8 promoter KO HoxB8 MPP and control with growth factors but without E2 ( ; ) at day 8. Gr1 + monocytes and CD11c + DC are shown. Quantification of Gr1 + monocytes of living single cells on day 3, 6, 8, and 10 of spontaneous DC differentiation. n=6, lncIrf8 promoter KO; n=4, control. Empty gRNA vector or non-targeting gRNA vector HoxB8 MPP were used as controls. Data represent mean ± SD of at least three independent experiments with different HoxB8 MPP clones of lncIrf8 promoter KO and control without deletion. *p<0.05, **p<0.01, ***p<0.001, multiple t-tests. Data that have no difference (p>0.05) are not labeled.

    Article Snippet: To further characterize the major transcripts of lncIrf8 , 3' and 5' end Rapid Amplification of cDNA Ends (RACE) PCR was performed using template-switching RT enzyme mix (New England Biolabs, M0466) and TA cloning kit (Thermo Fisher Scientific, K202020) (Appendix 1-key resources table).

    Techniques: RNA Sequencing Assay, ChIP-sequencing, Expressing, Control, Quantitative RT-PCR, Flow Cytometry, Microscopy, Plasmid Preparation, Clone Assay, Labeling

    Journal: eLife

    Article Title: A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation

    doi: 10.7554/eLife.83342

    Figure Lengend Snippet:

    Article Snippet: To further characterize the major transcripts of lncIrf8 , 3' and 5' end Rapid Amplification of cDNA Ends (RACE) PCR was performed using template-switching RT enzyme mix (New England Biolabs, M0466) and TA cloning kit (Thermo Fisher Scientific, K202020) (Appendix 1-key resources table).

    Techniques: Control, Over Expression, Activation Assay, Activity Assay, Recombinant, Saline, Expressing, Fluorescence, Reverse Transcription, Multiplex Assay, TA Cloning, Sequencing, Clone Assay, Capture-C, Software